I just started learning python and I'm experimenting new things.

isim = input("Name:")
soyad = input("Surname:")
yaş = input("Age:")
edu = input("Education:")
gen = input("Gender:")print("Name: {},Surname: {},Age: {},Education: {},Gender: {}".format(isim,soyad,yaş,edu,gen))

My aim is to use \n after all brackets ({}) so I'll be able to print them more organised but I don't want to use \n after every part.

print(a,b,c,d,e,sep = \n)

is the only way I know. I want to use sep and format together. How can I do that?

You're only printing one string created by using format so sep doesn't apply. You could expand a generator expression that adds "\n" to each argument to format, though.

print("Name: {},Surname: {},Age: {},Education: {},Gender: {}".format(*(f"{x}\n" for x in [isim,soyad,yaş,edu,gen])))

You may instead wish to use a multi-line f-string, though.

print(f"""Name: {isim}
Surname: {soyad}
Age: {yaş}
Education: {edu}
Gender: {gen}""")